• <tbody id="jprmy"></tbody><tbody id="jprmy"></tbody>

    <rp id="jprmy"><acronym id="jprmy"></acronym></rp>
  • <dd id="jprmy"><pre id="jprmy"></pre></dd>

    <tbody id="jprmy"><pre id="jprmy"></pre></tbody>

        <tbody id="jprmy"></tbody>
        <dd id="jprmy"><track id="jprmy"></track></dd>

        欢迎光临上海懋康生物科技有限公司
        主页 > 产品中心 > 生化试剂 > Rapamycin 雷帕霉素 > Rapamycin雷帕霉素
        产品中心
        Rapamycin雷帕霉素

        Rapamycin雷帕霉素

        简要描述:

        Rapamycin雷帕霉素用途及描述:科研试剂,广泛应用于分子生物学,药理学等科研方面。雷帕霉素(西罗莫司)是目前世界上的强效免疫抑制剂。

        产品时间:2016-09-28

        打印当前页

        分享到:

        Rapamycin雷帕霉素参数

        Rapamycin (Sirolimus) 雷帕霉素(西罗莫司)

        别  名

        西罗莫司(sirolimus)

         

         

        Cas 号

        53123-88-9

        分子式

        C51H79NO13  

        分子量

        914.18

        结构式

         

         

        Rapamycin雷帕霉素定义:

        一种新型大环内酯类免疫抑制药物。通过与相应免疫嗜素RMBP结合抑制细胞周期G0期和G1期,阻断G1进入S期而发挥作用,其效应为:①抑制T和B细胞增殖;②抑制IL-1、IL-2、IL-6和IFN-γ诱导的淋巴细胞增殖;③抑制IgG和供者特异性抗体(细胞毒抗体)产生;④抑制单核细胞增殖。可用于抗移植排斥反应和治疗类风湿性关节炎、红斑狼疮等自身免疫病。

        溶解性:可溶解于DMSO (50mg/ml)、甲醇(50 mg/ml)、乙醇、丙酮、等有机溶剂;几乎不溶于水。

        作用

        本品抑制由抗原和细胞因子(白介素IL-2、IL-4和IL-15)激发的T淋巴细胞的活化和增殖,它亦抑制抗体的产生。在细胞中,西罗莫司与免疫嗜素,即FK结合蛋白-12(FKBP-12)结合,生成FKBP-12免疫抑制复合物。此复合物与哺乳动物的西罗莫司BA分子(mTOR,一种关键的调节激酶)结合并抑制其活性,从而抑制细胞周期中G1期向S期的发展。

        Trusted Worldwide: more than 10,000 vials of our rapamycin have been shipped to more than 2,500 laboratories worldwide since 2002.

        Immunosuppressant, related to FK-506, but without calcineurin inhibitory activity even when complexed to FK-506 binding protein. Selectively blocks signaling that leads to p70 S6 kinase activation (IC50 = 50 pM). Terada, N., et al. "Failure of rapamycin to block proliferation once resting cells have entered the cell cycle despite inactivation of p70 S6 kinase." J. Biol. Chem. 268: 12062-12068 (1993). Fingar, D.C., et al. "Dissociation of pp70 ribosomal protein S6 kinase from insulin-stimulated glucose transport in 3T3-L1 adipocytes." J. Biol. Chem. 268: 3005-3008 (1993). Price, D.J., et al. "Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase." Science 257: 973-977 (1992). Chung, J., et al. "Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases." Cell 69: 1227-1236 (1992).

        Lymphokine-induced cell proliferation at the G1 phase is inhibited and apoptosis in a murine B cell line is induced by rapamycin. Rapamycin arrests the Saccharomyces cerevisiae cell cycle irreversibly in the G1 phase. Morice, W.G. ,et al. "Rapamycin-induced inhibition of p34cdc2 kinase activation is associated with G1/S-phase growth arrest in T lymphocytes." J. Biol. Chem. 268: 3734-3738 (1993). Kay, J.E., et al. "Inhibition of T and B lymphocyte proliferation by rapamycin." Immunology 72: 544-549 (1991). Heitman, J., et al. "Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast." Science 253: 905-909 (1991).

        Rapamycin extended median and maximal lifespan of both male and female mice when fed late in life. Harrison, D.E., et al. "Rapamycin fed late in life extends lifespan in genetically heterogeneous mice." Nature 460: 392-395 (2009).

        Rapamycin has shown activity in slowing cellular and organismal aging. Rapamycin abolished nuclear blebbing, delayed the start of cellular senescence, and improved the degradation of progerin in Hutchinson-Gilford progeria syndrome fibroblast cells. It also reduced the formation of insoluble progerin aggregates and resulted in clearance through autophagic mechanisms in normal fibroblasts. Cao, K., et al. "Rapamycin reverses cellular phenotypes and enhances mutant protein clearance in Hutchinson-Gilford progeria syndrome cells." Sci. Transl. Med. 3: 89ra58 (2011).

        Due to a different mechanism of action than FK506 and other immunosuppressants, rapamycin may prove to be important in organ transplant patient therapy. Fewer side effects than the standard anti-rejection treatments have been observed. Proliferation of activated T cells, but not apoptosis, is blocked by rapamycin. The induction of apoptosis of rejection-causing T cells reduces the tendency towards transplant rejection. Schwarz, C. and Oberbauer, R. "The future role of target of rapamycin inhibitors in renal transplantation." Curr Opin Urol. 12: 109-113 (2002). Wells, A.D. et al. "Requirement for T-cell apoptosis in the induction of peripheral transplantation tolerance." Nat. Med. 5: 1303-1307 (1999). Li, Y., et al. "Blocking both signal 1 and signal 2 of T-cell activation prevents apoptosis of alloreactive T cells and induction of peripheral allograft tolerance." Nat. Med. 5: 1298-1302 (1999).

        We have had one lot of Selleck Chemical's rapamycin analyzed by a highly experienced and expert clinical analytical laboratory that specializes in rapamycin analyses. Using liquid chromatography - mass spectrometry, they found a purity of 96.7% (cis plus trans) for a lot of Selleck's rapamycin. In contrast, we have proven our rapamycin to be greater than 99% in purity for every lot, no exceptions.

        More on the subject of rapamycin purity: HPLC analysis of rapamycin is somewhat complicated. Rapamycin forms several chromatographically separable species in solution, consisting perhaps of different conformers, tautomers, hydrates and/or isomers, but they are all in equilibrium with the major form, trans-rapamycin. We have shown this by collecting the individual impurity peaks in our rapamycin product and individually re-injecting them into the HPLC. In each case, upon re-injection the collected impurity peak is reduced or absent, and the major peak is again trans-rapamycin, of ~95% purity or higher, thus confirming re-equilibration back to the major trans-isomer of rapamycin. Because we find that all significant impurities (generally, those above 0.1%) in our product, including the cis-isomer, are in equilibrium with the trans isomer in the solution used for analysis, the actual purity of our product is >99% rapamycin, in all of its equilibrium forms. In contrast, material from other suppliers typically contains impurities that do not equilibrate with trans-rapamycin, and thus are genuine contaminants. These results also indicate that it is probably not possible to obtain the trans isomer in pure form, because in solution it will quickly re-equilibrate to the mixture of cis and trans.

        储存条件:-20℃,避光防潮密闭干燥。

         

        订购信息:

        品名

        产地

        货号

        规格

        单价

        备注

        Rapamycin (Sirolimus) 雷帕霉素(西罗莫司)

        MKbio

        MZ0201-10MG

        10MG

        248

        现货

        Rapamycin (Sirolimus) 雷帕霉素(西罗莫司)

        MKbio

        MZ0201-100MG

        100MG

        680

        现货

        上海懋康生物科技有限公司是一家涉足于生命科学和生物技术域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
        懋康生物精选*品牌和国内经过认证的、质量较为可靠、有质量体系管理的产品供应商的产品,能够根据课题需要,提供基于免疫学、分子生物学和细胞生物学,微生物学产品组合。通过进口产品和国内精选、质量可靠的产品组合,保证您的课题能顺利、甚至高质量完成。
        如果懋康生物没有您需要采购的产品,我们也会向您推荐优质供应商,让您自行选择和参考。懋康生物作为优秀的专业科研整体试剂服务提供商。汇聚了具有严格质控体系的著名试剂供应商及经验丰富的生物专业学术人员,致力于为中国医学与生物科研提供zui专业的生物试剂咨询导购,节省您的实验时间和经费,为您的论文发表保驾护航!

        特级胎牛血清(北美血源)

        GIBCO

        16000-044

        500ml

        特级胎牛血清(澳洲血源)

        GIBCO

        10099-141

        500ml

        普通型胎牛血清(南美血源)

        Hyclone

        SV30087.02

        500ml

        特级胎牛血清(澳洲血源)

        Hyclone

        SH30084.03

        500ml

        标准型胎牛血清(澳大利亚来源)

        Hyclone

        SH30370.03

        500ml

        特级胎牛血清(北美血源)

        Hyclone

        SH30070.03

        500ml

        胎牛血清  乌拉圭

        GIBCO

        C2027050

        500ml

        MSC QUALIFIED FBS

        GIBCO

        12662029

        500ml

        FBS, HI

        GIBCO

        10438026

        500ml

        FBS, ES CELL QUAL. USDA APPD.

        GIBCO

        10439024

        500ml

        FBS, DIALYZED, USA ORIGIN

        GIBCO

        26400044

        500ml

        FBS QUALIFIED, USA ORIGIN

        GIBCO

        26140079

        500ml

        FBS, ES CELL QUAL, USA ORIGIN

        GIBCO

        16141079

        500ml

        FBS, HI

        GIBCO

        16140071

        500ml

        巴西胎牛

        GIBCO

        12657029

        500ml

        新生牛血清

        GIBCO

        16010159

        500ml

        FOETAL BOVINE SERUM

        Hyclone

        SH30406.02

        500ml

        New Zealand Calf Serum

        Hyclone

        SH30401.01

        500ml

        FetalClone?II

        Hyclone

        SH30066.02

        100ml

        STANDARD FETAL BOVINE SERUM

        Hyclone

        SH30088.03

        500ml

        Newborn Bovine Calf Serum, U.S. Origin

        Hyclone

        SH30118.02

        100ml

        FETAL BOVINE SERUM

        Hyclone

        SH30070.02

        100ml

        COSMIC CALF?SERUM

        Hyclone

        SH30087.02

        100ml

        FETALBOVINESERUM CHARACTERIZSED

        Hyclone

        SH30070.03E

        500ml

        FetalClone?I

        Hyclone

        SH30080.03

        500ml

        FETAL BOVINE SERUM CHARACTERIZED

        Hyclone

        SH30396.03

        500ml

        FETAL BOVINE SERUM

        Hyclone

        SH30071.03

        500ml

        FETAL BOVINE SERUM

        Hyclone

        SH30071.02

        100ml

        FetalClone?I

        Hyclone

        SH30080.02

        100ml

        FETAL BOVINE SERUM CHARACTERIZSED

        Hyclone

        SH30070.02E

        100ml

        FETAL BOVINE SERUM

        Hyclone

        SH30079.02

        100ml

        CHARCOAL/DEXTRAN TREATED FBS

        Hyclone

        SH30068.02

        100ml

        CHARCOAL/DEXTRAN TREATED FBS

        Hyclone

        SH30068.03

        500ml

        FetalClone?II

        Hyclone

        SH30066.03

        500ml

        HyClone FetalClone?III

        Hyclone

        SH30109.03

        500ml

        STANDARD FETAL BOVINE SERUM

        Hyclone

        SH30088.03

        500ml

        STANDARD FETAL BOVINE SERUM

        Hyclone

        SH30088.02

        100ml

        FETAL BOVINE SERUM

        Hyclone

        SH30079.03

        500ml

        马血清

        Hyclone

        SH30074.03

        500ml

        1640培养基 1640 Medium

        GIBCO

        31800-022

        10X1L

        DMEM(高糖)含丙酮酸钠

        GIBCO

        12800-017

        10X1L

        DMEM(高糖) DMEM(High Glucose)

        GIBCO

        12100-046

        10X1L

        DMEM(低糖)DMEM(Low Glucose)

        GIBCO

        31600-034

        10X1L

        MEM 培养基

        GIBCO

        41500-034

        10X1L

        M199培养基 M199 Medium

        GIBCO

        31100-035

        10X1L

        F12培养基

        GIBCO

        21700-075

        10X1L

        DMEM/F12培养基

        GIBCO

        12400-024

        10X1L

        IMDM培养基

        GIBCO

        12200-036

        10X1L

        DMEM(高糖)培养液

        Hyclone

        SH30022.01B

        500ml

        DMEM(低糖) 培养液

        Hyclone

        SH30021.01B

        500ml

        1640培养液

        Hyclone

        SH30809.01B

        500ml

        DMEM/F12培养液

        Hyclone

        SH30023.01B

        500ml

        α-MEM培养液

        Hyclone

        SH30265.01B

        500ml

        MEM/EBSS培养液

        Hyclone

        SH30265.01B

        500ml

        胰蛋白酶/EDTA消化液

        Hyclone

        SV30042.01

        100ml

        青/链霉素浓缩液

        Hyclone

        SV30010

        100ml

        Lipofectamine? 2000 Transfection Reagent
         脂质体2000

        GIBCO

        11668-027

        0.75ml

        Lipofectamine? 2000 Transfection Reagent
         脂质体2000

        GIBCO

        11668-019

        1.5ml

        B-27? Serum-Free Supplement (50X), Liquid 
         

        GIBCO

        17504-044

        10ml

        N-2 Supplement (100X), Liquid
         

        GIBCO

        17502-048

        5ml

        G-5 Supplement (100X), Liquid 

        GIBCO

        17503-012

        1ml

        SUPERSCRIPT II

        invitrogen

        18064014

         

        SUPERSCRIPT III

        invitrogen

        18080044

         

        SUPERSCRIPT III 1ST

        invitrogen

        18080051

         

        Wizard? SV Gel and PCR Clean-Up System

        promega

        A9281

        50 preps

        Wizard? SV Gel and PCR Clean-Up System

        promega

        A9282

        250 preps

        Wizard? SV Gel and PCR Clean-Up System

        promega

        A9285

        1000 preps

        Dual-Glo? Luciferase Assay System

        promega

        E2920

        10ml

        Dual-Glo? Luciferase Assay System

        promega

        E2940

        100ml

        Dual-Glo? Luciferase Assay System

        promega

        E2980

        10 × 100ml

        Dual-Luciferase? Reporter Assay System

        promega

        E1910

        100 assays

        Dual-Luciferase? Reporter Assay System 10-Pack

        promega

        E1960

        1,000 assays

        Dual-Luciferase? Reporter 1000 Assay System

        promega

        E1980

        1,000 assays

        ImProm-II? Reverse Transcriptase

        promega

        A3803

        500 reactions

        ImProm-II? Reverse Transcription System

        promega

        A3800

        100 reactions

        Reverse Transcription System

        promega

        A3500

        100 reactions

        CellTiter 96? Non-Radioactive Cell Proliferation Assay

        promega

        G4000

        1,000 assays

        CellTiter 96? Non-Radioactive Cell Proliferation Assay

        promega

        G4100

        5,000 assays

        CellTiter 96? AQueous One Solution Cell Proliferation Assay

        promega

        G3580

        1,000 assays

        CellTiter 96? AQueous One Solution Cell Proliferation Assay

        promega

        G3581

        5,000 assays

        CellTiter 96? AQueous One Solution Cell Proliferation Assay

        promega

        G3582

        200 assays

        CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay

        promega

        G5421

        1,000 assays

        CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay

        promega

        G5430

        5,000 assays

        CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay

        promega

        G5440

        50,000 assays

        pGEM?-T Easy Vector System I

        promega

        A1360

        20 reactions

        pGEM?-T Easy Vector System II

        promega

        A1380

        20 reactions

        DeadEnd? Fluorometric TUNEL System

        promega

        G3250

        60 reactions

        DeadEnd? Colorimetric TUNEL System

        promega

        G7130

        40 reactions

        DeadEnd? Colorimetric TUNEL System

        promega

        G7360

        20 reactions

        M-MLV Reverse Transcriptase

        promega

        M1701

        10,000u

        M-MLV Reverse Transcriptase

        promega

        M1705

        50,000u

        GoTaq? qPCR Master Mix,

        promega

        A6001

        200 Reactions

        GoTaq? qPCR Master Mix,

        promega

        A6002

        1000 Reactions

        Wizard? DNA Clean-Up System

        promega

        A7280

        100 preps

        SV Total RNA Isolation System

        promega

        Z3100

        50 preps

        SV Total RNA Isolation System

        promega

        Z3101

        10 preps

        SV Total RNA Isolation System

        promega

        Z3105

        250 preps

        CellTiter-Glo? Luminescent Cell Viability Assay

        promega

        G7570

        10ml

        CellTiter-Glo? Luminescent Cell Viability Assay

        promega

        G7571

        10 × 10ml

        CellTiter-Glo? Luminescent Cell Viability Assay

        promega

        G7572

        100ml

        CellTiter-Glo? Luminescent Cell Viability Assay

        promega

        G7573

        10 × 100ml

        PepTag? Non-Radioactive PKC Assay

        promega

        V5330

        120 reactions

        PepTag? Non-Radioactive cAMP-Dependent Protein Kinase Assay

        promega

        V5340

        120 reactions

        留言框

        • 产品:

        • 您的单位:

        • 您的姓名:

        • 联系电话:

        • 常用邮箱:

        • 省份:

        • 详细地址:

        • 补充说明:

        • 验证码:

          请输入计算结果(填写阿拉伯数字),如:三加四=7
        • © 2019 上海懋康生物科技有限公司 版权所有 技术支持:环保在线
        • 电 话:18616957973 传 真:86-021-54736159 QQ号码:1563822900 邮 箱:2971634497@qq.com 地 址:上海市闵行区虹梅南路2588号绿亮科创园1幢A栋321室
        • 备案号:沪ICP备16016464号-3 总访问量:230943
        在线客服 联系方式

        服务热线

        18616957973

        jlzz日本人年轻护士_免费a级毛片_将春药推进闺蜜下面_极品呦女japanese